Proteins and Albumin

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2 . 4 . 1 Albumin and other carrier proteins

Soluble proteins make up 6.4–8.3% of plasma by weight [1], a value comparable to their concentration in cell water [2]. Many plasma proteins are synthesized by the liver. Their concentrations may decrease in the setting of liver disease, malnutrition or protein-losing states such as nephrotic syndrome, severe burns, bowel inflammation, intestinal lymphangiectasia or major bleeding episodes. The...

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Interaction of Phthalocyanine with Egg albumin and Bovine serum albumin

The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...

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Coelution of other proteins with albumin during size-exclusion HPLC: Implications for analysis of urinary albumin.

BACKGROUND Size-exclusion HPLC has been used as an alternative to immunoassays for quantifying urinary albumin (microalbumin). Systematically higher values for the HPLC method have been proposed to result from nonimmunoreactive albumin. METHODS We evaluated separation of purified proteins and urinary components by size-exclusion HPLC using a Zorbax Bio Series GF-250 column eluted with phospha...

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Identification of albumin-binding proteins in capillary endothelial cells

Isolated fat tissue microvessels and lung, whose capillary endothelia express in situ specific binding sites for albumin, were homogenized and subjected to SDS-gel electrophoresis and electroblotting. The nitrocellulose strips were incubated with either albumin-gold (Alb-Au) and directly visualized, or with [125I]albumin (monomeric or polymeric) and autoradiographed. The extracts of both microv...

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Albumin storage proteins in the protein bodies of castor bean.

Of the total protein in the protein bodies of castor bean (Ricinus communis L.), approximately 40% is represented by a group of closely related albumins localized in the matrix of the organelle. This group of albumins has a sedimentation value of 2S and is resolved into several proteins of molecular weight around 12,000 daltons by sodium dodecyl sulfate-acrylamide gel electrophoresis. It has a ...

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ژورنال

عنوان ژورنال: Laboratory Medicine

سال: 2014

ISSN: 0007-5027,1943-7730

DOI: 10.1309/lmkrnrgw5j03apzq